Date ( Creation )

2019-09-26

Identifier

http://data.bgs.ac.uk/id/dataHolding/13607536

Maintenance and update frequency

notApplicable notApplicable

GEMET - INSPIRE themes

• Geology

BGS Thesaurus of Geosciences

• Groundwater , Arsenic

Keywords

• NERC_DDC

Access constraints

otherRestrictions Other restrictions

Use constraints

otherRestrictions Other restrictions

Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Metadata language

English English

Topic category

• Geoscientific information

Geographic identifier

KAMPUCHEA [id=548000]

 

Date ( Creation )

1979

Geographic identifier

KANDAL [id=548780]

 

Date ( Creation )

1979

Distribution format

• .fastq files ()

OnLine resource

Data  

Hierarchy level

nonGeographicDataset Non geographic dataset

Other

non geographic dataset

Statement

Groundwater (500 mL) was collected in sterile plastic bottles (Corning), and filtered through a sterile 0.2 um nitrocellulose filter (Whatman). The filters were placed in DNA/RNA Shield Collection and Lysis Tubes (ZymoResearch) and homogenised using a Terralyzer (ZymoResearch) and transported to the UK. DNA was extracted from the samples, along with 3 negative controls, using the ZymoBIOMICS DNA Kit (ZymoResearch), according to the manufacturer's recommendations. A small volume (2 uL) from the extracted DNA was used to amplify the V4 hyper-variable region of the 16S rRNA gene by PCR, using a barcoded 515F and 806R primers, and the FastStart High Fidelity PCR System (Roche Diagnostics). The barcoded PCR products were purified and normalised to ~20 ng each, using the SequalPrep Normalization Kit (Fisher Scientific), pooled and sequenced on an Illumina MiSeq platform using V2 chemistry.

gmd:MD_Metadata

File identifier

95087060-b453-5bf3-e054-002128a47908   XML

Metadata language

English English

Hierarchy level

nonGeographicDataset Non geographic dataset

Hierarchy level name

non geographic dataset

Date stamp

2021-07-26

Metadata standard version

2.3

Dataset URI

http://data.bgs.ac.uk/id/dataHolding/13607536