16S rRNA Gene Sequencing of Filtered Groundwater from 8 Boreholes in Cambodia (NERC grant NE/P01304X/1)
This dataset represents the raw reads from sequencing the V4 hyper-variable region of the 16S rRNA gene on an Illumina MiSeq platform. The samples are filtered groundwater samples from 8 boreholes from a sandy-dominated site and a clay-dominated site in Cambodia that show arsenic concentrations above the WHO recommended limit, and were collected in May 2019.
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Citation proposal
. 16S rRNA Gene Sequencing of Filtered Groundwater from 8 Boreholes in Cambodia (NERC grant NE/P01304X/1). https://metadata.bgs.ac.uk/geonetwork/srv/api/records/95087060-b453-5bf3-e054-002128a47908 |
Simple
- Date ( Creation )
- 2019-09-26
Principal investigator
Originator
University of Manchester
-
Dr Naji Bassil
(School of Earth and Environmental Sciences )
Williamson Building
Manchester
M13 9PL
Distributor
Point of contact
- Maintenance and update frequency
- notApplicable notApplicable
- GEMET - INSPIRE themes
-
- Geology
- BGS Thesaurus of Geosciences
-
- Groundwater , Arsenic
- Keywords
-
- NERC_DDC
- Access constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
- Other constraints
- Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
- Metadata language
- English English
- Topic category
-
- Geoscientific information
- Geographic identifier
-
KAMPUCHEA [id=548000]
- Date ( Creation )
- 1979
- Geographic identifier
-
KANDAL [id=548780]
- Date ( Creation )
- 1979
Reference System Information
No information provided.
- Distribution format
-
- .fastq files ()
Distributor
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- non geographic dataset
Conformance result
- Date ( Publication )
- 2011
- Explanation
- See the referenced specification
- Pass
- false
Conformance result
- Date ( Publication )
- 2010-12-08
- Explanation
- See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
- Pass
- false
- Statement
- Groundwater (500 mL) was collected in sterile plastic bottles (Corning), and filtered through a sterile 0.2 um nitrocellulose filter (Whatman). The filters were placed in DNA/RNA Shield Collection and Lysis Tubes (ZymoResearch) and homogenised using a Terralyzer (ZymoResearch) and transported to the UK. DNA was extracted from the samples, along with 3 negative controls, using the ZymoBIOMICS DNA Kit (ZymoResearch), according to the manufacturer's recommendations. A small volume (2 uL) from the extracted DNA was used to amplify the V4 hyper-variable region of the 16S rRNA gene by PCR, using a barcoded 515F and 806R primers, and the FastStart High Fidelity PCR System (Roche Diagnostics). The barcoded PCR products were purified and normalised to ~20 ng each, using the SequalPrep Normalization Kit (Fisher Scientific), pooled and sequenced on an Illumina MiSeq platform using V2 chemistry.
gmd:MD_Metadata
- File identifier
- 95087060-b453-5bf3-e054-002128a47908 XML
- Metadata language
- English English
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- non geographic dataset
- Date stamp
- 2021-04-16
- Metadata standard version
- 2.3
Point of contact
British Geological Survey
Environmental Science Centre,Keyworth
NOTTINGHAM
NOTTINGHAMSHIRE
NG12 5GG
United Kingdom
- Dataset URI
- http://data.bgs.ac.uk/id/dataHolding/13607536