95087060-b453-5bf3-e054-002128a47908
English
nonGeographicDataset
non geographic dataset
British Geological Survey
+44 115 936 3100
Environmental Science Centre,Keyworth
NOTTINGHAM
NOTTINGHAMSHIRE
NG12 5GG
United Kingdom
enquiries@bgs.ac.uk
pointOfContact
2024-03-28
UK GEMINI
2.3
http://data.bgs.ac.uk/id/dataHolding/13607536
16S rRNA Gene Sequencing of Filtered Groundwater from 8 Boreholes in Cambodia (NERC grant NE/P01304X/1)
2019-09-26
creation
http://data.bgs.ac.uk/id/dataHolding/13607536
This dataset represents the raw reads from sequencing the V4 hyper-variable region of the 16S rRNA gene on an Illumina MiSeq platform. The samples are filtered groundwater samples from 8 boreholes from a sandy-dominated site and a clay-dominated site in Cambodia that show arsenic concentrations above the WHO recommended limit, and were collected in May 2019.
Prof Jonathan Lloyd
University of Manchester
School of Earth and Environmental Sciences
Manchester
M13 9PL
not available
principalInvestigator
Dr Naji Bassil
University of Manchester
School of Earth and Environmental Sciences
Williamson Building
Manchester
M13 9PL
not available
originator
Enquiries
British Geological Survey
not available
distributor
Enquiries
British Geological Survey
not available
pointOfContact
notApplicable
https://resources.bgs.ac.uk/images/geonetworkThumbs/95087060-b453-5bf3-e054-002128a47908.png
Geology
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Groundwater
NGDC Deposited Data
Arsenic
BGS Thesaurus of Geosciences
2022
revision
NGDC Deposited Data
dataCentre
NERC_DDC
otherRestrictions
licenceOGL
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
otherRestrictions
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
English
geoscientificInformation
British Geological Survey Gazetteer: Geographical hierarchy from Geosaurus
1979
creation
KAMPUCHEA [id=548000]
British Geological Survey Gazetteer: Geographical hierarchy from Geosaurus
1979
creation
KANDAL [id=548780]
2019-05-06
2019-05-12
.fastq files
Enquiries
British Geological Survey
not available
distributor
https://www.bgs.ac.uk/services/ngdc/accessions/index.html#item130306
Data
download
nonGeographicDataset
non geographic dataset
INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology
2011
publication
See the referenced specification
false
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
publication
See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
false
Groundwater (500 mL) was collected in sterile plastic bottles (Corning), and filtered through a sterile 0.2 um nitrocellulose filter (Whatman). The filters were placed in DNA/RNA Shield Collection and Lysis Tubes (ZymoResearch) and homogenised using a Terralyzer (ZymoResearch) and transported to the UK. DNA was extracted from the samples, along with 3 negative controls, using the ZymoBIOMICS DNA Kit (ZymoResearch), according to the manufacturer's recommendations. A small volume (2 uL) from the extracted DNA was used to amplify the V4 hyper-variable region of the 16S rRNA gene by PCR, using a barcoded 515F and 806R primers, and the FastStart High Fidelity PCR System (Roche Diagnostics). The barcoded PCR products were purified and normalised to ~20 ng each, using the SequalPrep Normalization Kit (Fisher Scientific), pooled and sequenced on an Illumina MiSeq platform using V2 chemistry.