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These data show images recorded using a variety of methods of a model system of bacterial metal reduction. In all cases the bacteria grew from a pure culture of Geobacter sulfurreducens, and grew undisturbed on thin films of amorphous Fe oxyhydroxide – ferrihydrite. The different imaging methodologies have highlighted different features of this interaction. AFM shows the surface texture of the bacteria and ferrihydrite films; epifluorescence was used to allow counting of the cells at different time points from 0 to 12 days post inoculation (cell counts available in excel spreadsheet); and confocal imaging allow visualisation of the redox patterns surrounding cells and to identify areas of bioreduced Fe(II) (quantification of Fe(II) available in excel spreadsheet). The following data is included: 1. 9 x AFM images of Geobacter sulfurreducens bacteria growing on ferrihydrite films 2. 5 x epifluorescence images of Geobacter sulfurreducens bacteria growing on ferrihydrite films over time 3. spreadsheet bacterial counts associated with epifluorescence images 4. 7 x confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with redox green staining of appendages 5. 5 x example confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with Fe(II) highlighted by RhoNox-1 6. Spreadsheet of quanitfication of RhoNox intensity against bacteria and Fe co-location Data is presented which shows the formation of precious metal nanoparticles on the surface of geobacter sulfurreducens cells. The images were produced by CryoTEM. Full details of the experiment are available in this publication http://onlinelibrary.wiley.com/doi/10.1002/ppsc.201600073/full 7. Powerpoint presentation of TEM images of precious metal nanoparticles formed on the surface of Geobacter cells
Loan IDA number - IDA271576. No data was obtained for microbial cultivation experiments with core samples SSK111460 and SSK111461 from UKGEOS Glasgow Observatory, borehole GGC01. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey. Attached document described methodology of enriching sandstone core (SSK111461) in different media types and shale core (SSK111460) in synthetic groundwater. No microbial growth was seen after 7 months.
EngD thesis describing experiments carried out using a variety of sandstones. This thesis investigates the microbial response to 10 ppm oxygen and 100ppm oxygen in experiments representing deep saline aquifer conditions. All experiments were conducted using sandstone, artificial groundwater and a microbial community designed to represent conditions found within deep saline aquifers. A microbial community, containing sulphate reducing bacteria, was isolated and identified from sandstone samples and then used in the experiments. The experiments were batch microcosms, a high pressure bioreactor and column flow experiments. Analyses of these experiments were conducted through gas analysis, water chemistry and DNA analysis from microbial communities.