Soil depth core collected from the Needle’s Eye site in Dumfries, Scotland. Clear plastic depth core was lowered into a bog within the site, excised, and capped at the top and bottom. Core was sliced at 1 cm intervals at the University of Manchester in an anaerobic bag. A total of 41 samples were generated. Soil samples were returned to Newcastle University.

Two sediment depth cores were collected from a mud sediment patch at the sea floor of the Irish Sea. Cores were collected by the University of Manchester. Cores were sliced at 1 cm intervals from 0 - 10 cm, and at 2 cm intervals thereafter. Slicing was performed in an anaerobic bag. Samples were transferred to Newcastle University for DNA extraction. A total of 21 samples were extracted for core 1, and 23 samples extracted for core 2.

Two sediment depth cores were collected from the floor of Loch Etive, near Oban, Scotland. Slicing was performed in an anaerobic bag. Samples were taken at 0.5 cm increment between 0 - 2 cm, 1 cm increments between 2 - 10 cm, and at either 2 or 5 cm increments thereafter. Samples were transferred to Newcastle University for DNA extraction. A total of 21 samples were extracted for core 1, and 23 samples extracted for core 2.