c5f78e0e-2f47-7141-e054-002128a47908 English dataset British Geological Survey +44 115 936 3100 Environmental Science Centre,Keyworth NOTTINGHAM NOTTINGHAMSHIRE NG12 5GG United Kingdom enquiries@bgs.ac.uk pointOfContact 2026-03-05 UK GEMINI 2.3 http://data.bgs.ac.uk/id/dataHolding/13607775 OSGB 1936 / British National Grid (EPSG::27700) Microbial DNA extraction and PCR from core samples SSK111456 and SSK111457 from UKGEOS Glasgow Observatory borehole GGC01 2021-06-22 creation http://data.bgs.ac.uk/id/dataHolding/13607775 Loan IDA number IDA271576. Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent PCR from shale and sandstone core samples collected and preserved for microbiology (shale SSK111456 and sandstone SSK111457) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey. Katie Edwards University of Bristol PhD Student School of Earth Sciences Wills Memorial Building, Queens Road, Clifton Bristol BS8 1RJ not available originator Enquiries British Geological Survey United Kingdom not available distributor Enquiries British Geological Survey United Kingdom not available pointOfContact notApplicable https://resources.bgs.ac.uk/images/geonetworkThumbs/c5f78e0e-2f47-7141-e054-002128a47908.png Geology GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Geomicrobiology Sandstone Shale BGS Thesaurus of Geosciences 2022 revision NGDC Deposited Data Scottish SDI UKGEOS dataCentre NERC_DDC otherRestrictions licenceOGL Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]" otherRestrictions The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused. Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]" textTable English geoscientificInformation British Geological Survey Gazetteer: OS gazetteer 2002 revision Glasgow [id=1298677] -4.2216 -4.2216 55.8411 55.8411 2020-03-01 2020-08-31 Microsoft Excel witheld Enquiries British Geological Survey United Kingdom not available distributor https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item166985 WWW:DOWNLOAD-1.0-http--download Data download dataset dataset INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology 2011 publication See the referenced specification false Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF false Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.