a0043087-00a3-2ad1-e054-002128a47908
English
dataset
British Geological Survey
+44 115 936 3100
Environmental Science Centre,Keyworth
NOTTINGHAM
NOTTINGHAMSHIRE
NG12 5GG
United Kingdom
enquiries@bgs.ac.uk
pointOfContact
2024-03-27
UK GEMINI
2.3
http://data.bgs.ac.uk/id/dataHolding/13607593
Needle's Eye Depth Core Ion Torrent FASTQ Data (NERC Grant NE/L000326/1)
2020-02-21
creation
http://data.bgs.ac.uk/id/dataHolding/13607593
Soil depth core collected from the Needle’s Eye site in Dumfries, Scotland. Clear plastic depth core was lowered into a bog within the site, excised, and capped at the top and bottom. Core was sliced at 1 cm intervals at the University of Manchester in an anaerobic bag. A total of 41 samples were generated. Soil samples were returned to Newcastle University.
Peter Leary
University of Newcastle
Bioinformatics Support Unit
Leech Building, Faculty of Medical Sciences, Framlington Place
Newcastle
NE2 4HH
not available
pointOfContact
Peter Leary
University of Newcastle
Bioinformatics Support Unit
Leech Building, Faculty of Medical Sciences, Framlington Place
Newcastle
NE2 4HH
not available
principalInvestigator
notApplicable
https://resources.bgs.ac.uk/images/geonetworkThumbs/a0043087-00a3-2ad1-e054-002128a47908.png
Geology
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Bacteria
Uranium
NGDC Deposited Data
BGS Thesaurus of Geosciences
2022
revision
NGDC Deposited Data
dataCentre
NERC_DDC
otherRestrictions
licenceOGL
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
otherRestrictions
The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
English
geoscientificInformation
British Geological Survey Gazetteer: OS Boundary Line
2009
revision
Dumfries and Galloway [id=31903]
-3.6800
-3.6800
54.8800
54.8800
2014-12-02
2014-12-02
FASTQ file
https://www.bgs.ac.uk/services/ngdc/accessions/index.html#item132905
Data
download
dataset
dataset
INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology
2011
publication
See the referenced specification
false
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
publication
See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
false
DNA was extracted using FastDNA SPIN Kit for Soil. PCR using the 515F and 926R primers targeting the V4-V5 region of the 16S rRNA gene. Amplicon length was 481 bp. PCRs were 25 uL reactions with 22 uL of MegaMix Blue, 1uL of forward and 1 uL of reverse primer at 100 pM concentration, and 1 uL of 1:10 diluted template DNA. Initial denaturation was 95C for 5 minutes, followed by 30 cycles of 95C for 1 minute, 55C for 1 minutes, 72C for 1 minute, and a final elongation step of 72C for 10 minutes. Each depth sample was run in triplicate and pooled. Pooled amplicons were cleaned using Agencourt AMPure XP. PCR amplicons were quantified via Qubit 3.0 fluorometer and a total dilution factor was calculated for each sample, diluting each sample to 1000. Equimolar samples were pooled into a final library. Sequencing was performed on the Ion Torrent PGM on a 316 chip.