Date ( Creation )

2020-02-21

Identifier

http://data.bgs.ac.uk/id/dataHolding/13607591

Maintenance and update frequency

notApplicable notApplicable

GEMET - INSPIRE themes

• Geology

BGS Thesaurus of Geosciences

• Core samples , Bacteria , Uranium

Keywords

• NERC_DDC

Access constraints

otherRestrictions Other restrictions

Use constraints

otherRestrictions Other restrictions

Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

Either: (i) the dataset is made freely available, e.g. via the Internet, for a restricted category of use (e.g. educational use only); or (ii) the dataset has not been formally approved by BGS for access and use by external clients under licence, but its use may be permitted under alternative formal arrangements; or (iii) the dataset contains 3rd party data or information obtained by BGS under terms and conditions that must be consulted in order to determine the permitted usage of the dataset. Refer to the BGS staff member responsible for the creation of the dataset if further advice is required. He / she should be familiar with the composition of the dataset, particularly with regard to 3rd party IPR contained in it, and any resultant use restrictions. This staff member should revert to the IPR Section (ipr@bgs.ac.uk ) for advice, should the position not be clear.

Spatial representation type

textTable Text, table

Metadata language

English English

Topic category

• Geoscientific information

Geographic identifier

Ravenglass [id=1458961]

 

Date ( Revision )

2002

Distribution format

• fastq ()

OnLine resource

https://www.bgs.ac.uk/services/ngdc/accessions/index.html#item132965  

Hierarchy level

dataset Dataset

Other

dataset

Statement

DNA was extracted using FastDNA SPIN Kit for Soil. PCR using the 515F and 926R primers targeting the V4-V5 region of the 16S rRNA gene. Amplicon length was 481 bp. PCRs were 25 uL reactions with 22 uL of MegaMix Blue, 1uL of forward and 1 uL of reverse primer at 100 pM concentration, and 1 uL of 1:10 diluted template DNA. Initial denaturation was 95C for 5 minutes, followed by 30 cycles of 95C for 1 minute, 55C for 1 minutes, 72C for 1 minute, and a final elongation step of 72C for 10 minutes. Each depth sample was run in triplicate and pooled. Pooled amplicons were cleaned using Agencourt AMPure XP. PCR amplicons were quantified via Qubit 3.0 fluorometer and a total dilution factor was calculated for each sample, diluting each sample to 1000. Equimolar samples were pooled into a final library. Sequencing was performed on the Ion Torrent PGM on a 316 chip.

gmd:MD_Metadata

File identifier

9f8e367b-d26f-3fa5-e054-002128a47908   XML

Metadata language

English English

Hierarchy level

dataset Dataset

Date stamp

2021-04-16

Metadata standard version

2.3

Dataset URI

http://data.bgs.ac.uk/id/dataHolding/13607591