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Needle's Eye Depth Core (Control) Ion Torrent FASTQ Data (NERC Grant NE/L000326/1)

Soil depth core collected from the Needle’s Eye site in Dumfries, Scotland. Clear plastic depth core was lowered into a bog within the site, excised, and capped at the top and bottom. Core was sliced at 1 cm intervals at the University of Manchester in an anaerobic bag. A total of 42 samples were generated. Soil samples were returned to Newcastle University.

Simple

Date (Creation)
2020-02-21
Citation identifier
http://data.bgs.ac.uk/id/dataHolding/13607590
Point of contact
Organisation name Individual name Electronic mail address Role

University of Newcastle

Peter Leary

not available

Originator

University of Newcastle

Dr Neil Gray

not available

Principal investigator

British Geological Survey

Enquiries

not available

Distributor

British Geological Survey

Enquiries

not available

Point of contact
Maintenance and update frequency
notApplicable

GEMET - INSPIRE themes, version 1.0

  • Geology

BGS Thesaurus of Geosciences

  • Uranium

  • Bacteria

  • NGDC Deposited Data

  • Core samples

dataCentre
  • NGDC Deposited Data
Keywords
  • NERC_DDC

Access constraints
Other restrictions
Other constraints
licenceOGL
Other constraints
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
Use constraints
Other restrictions
Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Language
English
Topic category
  • Geoscientific information
Geographic identifier
Dumfries [id=1279734]

British Geological Survey Gazetteer: OS gazetteer 2002 revision

N
S
E
W
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Begin date
2014-12-02
End date
2014-12-02

Reference System Information

No information provided.
Distribution format
Name Version

fastq

Distributor contact
Organisation name Individual name Electronic mail address Role

British Geological Survey

Enquiries

not available

Distributor
OnLine resource
Protocol Linkage Name
https://www.bgs.ac.uk/services/ngdc/accessions/index.html#item132887

Data

Hierarchy level
Dataset
Other

dataset

Conformance result

Title

INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology

Date (Publication)
2011
Explanation

See the referenced specification

Pass
No

Conformance result

Title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication)
2010-12-08
Explanation

See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass
No
Statement

DNA was extracted using FastDNA SPIN Kit for Soil. PCR using the 515F and 926R primers targeting the V4-V5 region of the 16S rRNA gene. Amplicon length was 481 bp. PCRs were 25 uL reactions with 22 uL of MegaMix Blue, 1uL of forward and 1 uL of reverse primer at 100 pM concentration, and 1 uL of 1:10 diluted template DNA. Initial denaturation was 95C for 5 minutes, followed by 30 cycles of 95C for 1 minute, 55C for 1 minutes, 72C for 1 minute, and a final elongation step of 72C for 10 minutes. Each depth sample was run in triplicate and pooled. Pooled amplicons were cleaned using Agencourt AMPure XP. PCR amplicons were quantified via Qubit 3.0 fluorometer and a total dilution factor was calculated for each sample, diluting each sample to 1000. Equimolar samples were pooled into a final library. Sequencing was performed on the Ion Torrent PGM on a 316 chip.

Metadata

File identifier
9f8d7782-daf2-2a82-e054-002128a47908 XML
Metadata language
English
Hierarchy level
Dataset
Date stamp
2025-05-08
Metadata standard name
UK GEMINI
Metadata standard version

2.3

Metadata author
Organisation name Individual name Electronic mail address Role

British Geological Survey

enquiries@bgs.ac.uk

Point of contact
Dataset URI

http://data.bgs.ac.uk/id/dataHolding/13607590

 
 

Overviews

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Spatial extent

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Keywords

NGDC Deposited Data


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