Identification info

Metadata Language

English (en)

Dataset Reference Date ()

2020-02-21

Identifier

http://data.bgs.ac.uk/id/dataHolding/13607590

Maintenance and update frequency

notApplicable

GEMET - INSPIRE themes

• Geology

BGS Thesaurus of Geosciences

• Uranium

• Bacteria

• Core samples

Keywords

• NERC_DDC

Limitations on Public Access

otherRestrictions

Other constraints

licenceOGL

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Use constraints

otherRestrictions

Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Topic category

• Geoscientific information

Extent

Dumfries [id=1279734]

Dataset Reference Date ()

2002

Begin date

2014-12-02

End date

2014-12-02

 

Distribution Information

Data format

• fastq

  ()

 

Quality Scope

dataset

Other

dataset

Statement

DNA was extracted using FastDNA SPIN Kit for Soil. PCR using the 515F and 926R primers targeting the V4-V5 region of the 16S rRNA gene. Amplicon length was 481 bp. PCRs were 25 uL reactions with 22 uL of MegaMix Blue, 1uL of forward and 1 uL of reverse primer at 100 pM concentration, and 1 uL of 1:10 diluted template DNA. Initial denaturation was 95C for 5 minutes, followed by 30 cycles of 95C for 1 minute, 55C for 1 minutes, 72C for 1 minute, and a final elongation step of 72C for 10 minutes. Each depth sample was run in triplicate and pooled. Pooled amplicons were cleaned using Agencourt AMPure XP. PCR amplicons were quantified via Qubit 3.0 fluorometer and a total dilution factor was calculated for each sample, diluting each sample to 1000. Equimolar samples were pooled into a final library. Sequencing was performed on the Ion Torrent PGM on a 316 chip.

Metadata

File identifier

9f8d7782-daf2-2a82-e054-002128a47908 XML

Metadata Language

English (en)

Resource type

dataset

Metadata Date

2023-03-23

Metadata standard name

UK GEMINI

Metadata standard version

2.3