The amino acid composition of 20 aragonite samples precipitated in vitro in the presence and absence of amino acid (aspartic acid and glycine, separately and in combination), the dipeptide glycyl-L-aspartic acid and tetra-aspartic acid.

Amino acid compositions of aragonite samples precipitated from seawater, using a pH stat titrator using the constant composition technique between September 2021 and December 2022. Samples were precipitated from 330 mL of seawater with no biomolecules (control) or with a seawater concentration of 2 mM of aspartic acid (Asp), 2 mM glycine (Gly), 2 mM of both amino acids (Asp+Gly) or 2 mM dipeptide glycyl-L-aspartic acid (Asp-Gly) or from 33 mL of seawater with variable concentrations of aspartic acid (Asp) or tetra-aspartic acid (Asp4). Protein was extracted from the samples and run as free amino acids (to detect amino acids in free form) and as hydrolysed samples (to detect peptides). Data were collected to determine how changes in the calcification fluids of calcareous organisms affect aragonite precipitation. Data were collected by Giacomo Gardella, Sam Presslee and Nicola Allison and interpreted by Giacomo Gardella, Cristina Castillo Alvarez, Nicola Allison, Adrian Finch, Kirsty Penkman, Roland Krӧger, Sam Presslee and Matthieu Clog

Date (Creation): 2024-10-30

Citation identifier: http://data.bgs.ac.uk/id/dataHolding/13608300

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of St Andrews	Nicola Allison	not available	Originator
British Geological Survey	Enquiries	not available	Distributor
British Geological Survey	Enquiries	not available	Point of contact

Maintenance and update frequency: notApplicable

GEMET - INSPIRE themes, version 1.0

Geology

BGS Thesaurus of Geosciences

NGDC Deposited Data
Calcification
Aragonite
Sea water

dataCentre

NGDC Deposited Data

Keywords

NERC_DDC

Access constraints: Other restrictions

Other constraints: licenceOGL

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Use constraints: Other restrictions

Other constraints: The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints: Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Language: English

Topic category

Geoscientific information

Begin date: 2021-09-01

End date: 2022-12-23

Reference System Information

No information provided.

Distribution format

Name	Version
CSV

Distributor contact

Organisation name	Individual name	Electronic mail address	Role
British Geological Survey	Enquiries	not available	Distributor

OnLine resource

Protocol	Linkage	Name
	https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item186472	Data

Hierarchy level: Non geographic dataset

Other: non geographic dataset

Conformance result

Title: INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology

Date (Publication): 2011

Explanation: See the referenced specification

Pass: No

Conformance result

Title: Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication): 2010-12-08

Explanation: See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass: No

Statement: Artificial seawater was manipulated to alter pH and dissolved inorganic carbon concentration and the biomolecule, if used, was added. An aragonite seed was added as a surface for aragonite growth. A pH stat titrator monitored pH and dosed titrants (Na2CO3 and CaCl2) to replace the Ca2+ and CO32- consumed during aragonite formation. At the end, the solid was collected by filtration, rinsed with water and ethanol, dried at 40°C and stored. For amino acid analysis, samples were bleached, rinsed, demineralised and characterised by reverse phase HPLC in both free and hydrolysed form.