QICS Data: Gene expression data of scallops Pecten maximus and mussels Mytilus edulis held in the vicinity of a sub-seabed CO2 release (2012)

The QICS project (Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbons Storage) was established to improve our understanding of the potential impacts of CO2 release on the environment and to develop tools and best practice for monitoring sub-seabed CCS reservoirs. To monitor the potential impact of a CO2 leak to surficial benthic megafauna, cages of bivalves (the common mussel Mytilus edulis Linnaeus, 1758 and the king scallop Pecten maximus (Linnaeus, 1758)) were deployed at the gas release site and at a reference site in the QICS experiment - both within Ardmucknish Bay, Oban, Scotland. Replicate individuals were sampled at six time points over a 125-day period, which spanned both the 37-day injection and recovery phases of the experiment, in order to establish impacts to molecular physiology. Samples of bivalves were also simultaneously sampled from a reference site within the bay in order to contrast changes in physiology induced by the gas release with naturally variability in the physiological performance of both species. There was no evidence of gene regulation of either selected carbonic anhydrases (CAx genes) or the alpha subunit of sodium potassium ATPAses (ATP1A genes) in individual bivalves collected from the CO2 gas release site, in either species. In the common mussel Mytilus edulis there was only evidence for changes with time in the expression of genes coding for different classes of carbonic anhydrase. It was concluded that the effects of the plume of elevated pCO2 on ion-regulatory gene transcription were negligible in both species. Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.

Date (Creation): 2012

Citation identifier: http://data.bgs.ac.uk/id/dataHolding/13606457

Point of contact

Organisation name	Individual name	Electronic mail address	Role
National Oceanography Centre	Chris Hauton	not available	Point of contact
National Oceanography Centre	Chris Hauton	not available	Principal investigator

Maintenance and update frequency: notApplicable

GEMET - INSPIRE themes, version 1.0

Geology

BGS Thesaurus of Geosciences

Monitoring
Environmental impact
Carbon
UKCCS
NGDC Deposited Data
Storage

dataCentre

Keywords

NERC_DDC

Access constraints: Other restrictions

Other constraints: intellectualPropertyRights

Other constraints: Either : (i) the dataset has not been formally approved by BGS for access and use by external clients under license; and / or (ii) the dataset contains 3rd party data or information obtained by BGS under terms and conditions that must be consulted before the dataset can be provided to, or accessed by, BGS staff or external clients. Refer to the BGS staff member responsible for the creation of the dataset if further advice is required. He / she should be familiar with the composition of the dataset, particularly with regard to 3rd party IPR contained in it, and any resultant access restrictions. This staff member should revert to the IPR Section (IPR@bgs.ac.uk) for advice, should the position not be clear.

Use constraints: Other restrictions

Other constraints: The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints: Either: (i) the dataset is made freely available, e.g. via the Internet, for a restricted category of use (e.g. educational use only); or (ii) the dataset has not been formally approved by BGS for access and use by external clients under licence, but its use may be permitted under alternative formal arrangements; or (iii) the dataset contains 3rd party data or information obtained by BGS under terms and conditions that must be consulted in order to determine the permitted usage of the dataset. Refer to the BGS staff member responsible for the creation of the dataset if further advice is required. He / she should be familiar with the composition of the dataset, particularly with regard to 3rd party IPR contained in it, and any resultant use restrictions. This staff member should revert to the IPR Section ( ipr@bgs.ac.uk) for advice, should the position not be clear.

Language: English

Topic category

Geoscientific information

Geographic identifier: Ardmucknish Bay [id=1214829]
British Geological Survey Gazetteer: OS gazetteer 2002 revision

Begin date: 2012-05-08

End date: 2012-09-09

Supplemental Information: Pratt et al. 2015. No evidence for impacts to the molecular ecophysiology of ion or CO2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO2 release. International Journal of Greenhouse Gas Control. DOI:10.1016/j.ijggc.2014.10.001. QICS project website: www.bgs.ac.uk/qics/home.html.

Reference System Information

No information provided.

Distribution format

Name	Version
.xls

OnLine resource

Protocol	Linkage	Name
	http://www.bgs.ac.uk/ukccs/accessions/index.html#item8561

Hierarchy level: Dataset

Other: dataset

Conformance result

Title: INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology

Date (Publication): 2011

Explanation: See the referenced specification

Pass: No

Conformance result

Title: Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication): 2010-12-08

Explanation: See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass: No

Statement: Peer-reviewed paper to be published in the International Journal of Greenhouse Gas Control (in press). Will include original gene expression values and details of processing (citation to follow). Organism sampling, including tissue dissections conducted by Chris Hauton and Elizabeth Morgan; Ocean and Earth Science, University of Southampton Molecular methods conducted by Nicola Pratt; Ocean and Earth Science, University of Southampton This spreadsheet compiled by Hauton and Pratt, Ocean and Earth Science, University of Southampton. RNA extraction using TRIreagent (SigmaAldrich, Dorset, UK) according to manufacturer's instructions (Product No. T9424) RNA pellets air dried and diluted in DEPC-treated water (DEPC = diethyl pyrocarbonate) RNA purity assessed by measuring absorbance at 230nm, 260nm and 280nm using a NanoDrop spectrophotometer RNA quantity assessed using Bio-Rad (Herts, UK) Experion electrophoresis system according to manufacturer's instructions (Product No. 700-7104) RNA integrity (RQI) assessed using Bio-Rad Experion electrophoresis system according to manufacturer's instructions RNA DNase-treated using Promega (Hants, UK) RQ1 RNase-Free DNase, according to manufacturer's instructions (Product No. M6101) cDNA produced using LifeTechnologies (Strathclyde, UK) Superscript III Reverse Transcriptase according to manufacturer's instructions with oligo-dT priming (Product No. 18080044) qPCR carried out using a Qiagen (West Sussex) Rotorgene 3000 instrument qPCR Mastermix - Precision SY Mastermix with SYBR green (PrimerDesign, Hants, UK) (Product No. Precision-SY). qPCR conditions: 95 degrees for 10 minutes; 40 cycles of 95 degrees for 10 seconds, 60 degrees for 60 seconds qPCR amplicon specificity verified by melt curve analysis qPCR run files available upon request to Chris Hauton ( ch10@noc.soton.ac.uk) Acknowledgements: field sampling and molecular lab work funded under the UK NERC QICS Project (NE/H013881/1)