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Microbial DNA extraction and PCR from core samples SSK105556 and SSK105560 from UKGEOS Glasgow Observatory, borehole GGC01

Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.

Simple

Date (Creation)
2023-08-03
Citation identifier
http://data.bgs.ac.uk/id/dataHolding/13608095
Point of contact
Organisation name Individual name Electronic mail address Role

University of Bristol

Katie Edwards

not available

Originator

British Geological Survey

Enquiries

not available

Distributor

British Geological Survey

Enquiries

not available

Point of contact
Maintenance and update frequency
notApplicable

GEMET - INSPIRE themes, version 1.0

  • Geology

BGS Thesaurus of Geosciences

  • Abrasion

  • Geomicrobiology

  • NGDC Deposited Data

  • Scottish SDI

  • Sandstone

  • Shale

dataCentre
  • NGDC Deposited Data
  • Scottish SDI
  • UKGEOS
Keywords
  • NERC_DDC

Access constraints
Other restrictions
Other constraints
licenceOGL
Other constraints
Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"
Use constraints
Other restrictions
Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

There are no restrictions on the use that may be made of the dataset, although an appropriate copyright acknowledgement must be made when any part of the dataset is reproduced. Either no third party data / information is contained in the dataset or BGS has secured written permission from the owner of any third party data / information contained in the dataset to make the dataset freely available without any use constraints - inclusion of any third party data / information will affect the copyright acknowledgement that needs to be made.

Spatial representation type
Vector
Language
English
Topic category
  • Geoscientific information
Geographic identifier
Glasgow [id=1298677]

British Geological Survey Gazetteer: OS gazetteer 2002 revision

N
S
E
W
thumbnail




Begin date
2021-06-01
End date
2021-06-30
Unique resource identifier
OSGB 1936 / British National Grid (EPSG::27700)
Distribution format
Name Version

MS Excel

MS Word

Distributor contact
Organisation name Individual name Electronic mail address Role

British Geological Survey

Enquiries

not available

Distributor
OnLine resource
Protocol Linkage Name
https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item181843

Data

Hierarchy level
Dataset
Other

dataset

Conformance result

Title

INSPIRE Implementing rules laying down technical arrangements for the interoperability and harmonisation of Geology

Date (Publication)
2011
Explanation

See the referenced specification

Pass
No

Conformance result

Title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication)
2010-12-08
Explanation

See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass
No
Statement

Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.

Metadata

File identifier
027b4472-e717-1de8-e063-0937940a5dd1 XML
Metadata language
English
Hierarchy level
Dataset
Date stamp
2025-05-08
Metadata standard name
UK GEMINI
Metadata standard version

2.3

Metadata author
Organisation name Individual name Electronic mail address Role

British Geological Survey

enquiries@bgs.ac.uk

Point of contact
Dataset URI

http://data.bgs.ac.uk/id/dataHolding/13608095

 
 

Overviews

overview

Spatial extent

thumbnail

Keywords

NGDC Deposited Data Scottish SDI UKGEOS


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